In vitro degradation of natural insoluble lignin in aqueous media by the extracellular peroxidases of Phanerochaete chrysosporium.
Identifieur interne : 000B71 ( Main/Exploration ); précédent : 000B70; suivant : 000B72In vitro degradation of natural insoluble lignin in aqueous media by the extracellular peroxidases of Phanerochaete chrysosporium.
Auteurs : D N Thompson [États-Unis] ; B R Hames ; C A Reddy ; H E GrethleinSource :
- Biotechnology and bioengineering [ 0006-3592 ] ; 1998.
Descripteurs français
- KwdFr :
- Bioréacteurs (MeSH), Chromatographie gazeuse-spectrométrie de masse (MeSH), Dialyse (méthodes), Dépollution biologique de l'environnement (MeSH), Eau (MeSH), Lignine (composition chimique), Lignine (métabolisme), Logiciel (MeSH), Matrice extracellulaire (enzymologie), Peroxidases (composition chimique), Peroxidases (métabolisme), Phanerochaete (enzymologie), Solubilité (MeSH), Spectroscopie infrarouge à transformée de Fourier (méthodes), Traitement d'image par ordinateur (MeSH).
- MESH :
- composition chimique : Lignine, Peroxidases.
- enzymologie : Matrice extracellulaire, Phanerochaete.
- métabolisme : Lignine, Peroxidases.
- méthodes : Dialyse, Spectroscopie infrarouge à transformée de Fourier.
- Bioréacteurs, Chromatographie gazeuse-spectrométrie de masse, Dépollution biologique de l'environnement, Eau, Logiciel, Solubilité, Traitement d'image par ordinateur.
English descriptors
- KwdEn :
- Biodegradation, Environmental (MeSH), Bioreactors (MeSH), Dialysis (methods), Extracellular Matrix (enzymology), Gas Chromatography-Mass Spectrometry (MeSH), Image Processing, Computer-Assisted (MeSH), Lignin (chemistry), Lignin (metabolism), Peroxidases (chemistry), Peroxidases (metabolism), Phanerochaete (enzymology), Software (MeSH), Solubility (MeSH), Spectroscopy, Fourier Transform Infrared (methods), Water (MeSH).
- MESH :
- chemical , chemistry : Lignin, Peroxidases.
- enzymology : Extracellular Matrix, Phanerochaete.
- chemical , metabolism : Lignin, Peroxidases.
- methods : Dialysis, Spectroscopy, Fourier Transform Infrared.
- Biodegradation, Environmental, Bioreactors, Gas Chromatography-Mass Spectrometry, Image Processing, Computer-Assisted, Software, Solubility, Water.
Abstract
The lignin peroxidases (LIP) and manganese peroxidases (MNP) of Phanerochaete chrysosporium catalyze a wide range of lignin depolymerization reactions with lignin models and synthetic lignins in solution. However, their ability to degrade insoluble natural lignin in aqueous media has not been demonstrated. Insoluble isolated poplar lignin similar to natural lignin was treated in vitro in aqueous media for 12 h with LIP, MNP, and both. Treatment with MNP alone slightly increased the solid mass and produced measurable amounts of lignin-derived 2,6-dimethoxyhydroquinone and 2-methoxyhydroquinone but did not appreciably decrease the total lignin content. Treatment with LIP alone did not decrease the mass but produced measurable amounts of lignin-derived p-hydroxybenzoic acid and slightly decreased the lignin content. Finally, treatment with LIP and MNP together decreased the solid mass by 11%, decreased the lignin content by 5%, and released low-concentration compounds with mass spectra containing the typical lignin-derived electron-impact fragments of mass 107, 137, 151, 167, and 181. These results suggest that MNP increases the effectiveness of LIP-mediated lignin degradation.
DOI: 10.1002/(sici)1097-0290(19980320)57:6<704::aid-bit8>3.0.co;2-p
PubMed: 10099250
Affiliations:
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sortKey="Reddy, C A" sort="Reddy, C A" uniqKey="Reddy C" first="C A" last="Reddy">C A Reddy</name></author><author><name sortKey="Grethlein, H E" sort="Grethlein, H E" uniqKey="Grethlein H" first="H E" last="Grethlein">H E Grethlein</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1998">1998</date><idno type="RBID">pubmed:10099250</idno><idno type="pmid">10099250</idno><idno type="doi">10.1002/(sici)1097-0290(19980320)57:6<704::aid-bit8>3.0.co;2-p</idno><idno type="wicri:Area/Main/Corpus">000B52</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000B52</idno><idno type="wicri:Area/Main/Curation">000B52</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000B52</idno><idno type="wicri:Area/Main/Exploration">000B52</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">In vitro degradation of natural insoluble lignin in aqueous media by the extracellular peroxidases of Phanerochaete chrysosporium.</title><author><name sortKey="Thompson, D N" sort="Thompson, D N" uniqKey="Thompson D" first="D N" last="Thompson">D N Thompson</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemical Engineering, Michigan State University, East Lansing, Michigan 48824, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical Engineering, Michigan State University, East Lansing, Michigan 48824</wicri:regionArea><orgName type="university">Université d'État du Michigan</orgName><placeName><settlement type="city">East Lansing</settlement><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Hames, B R" sort="Hames, B R" uniqKey="Hames B" first="B R" last="Hames">B R Hames</name></author><author><name sortKey="Reddy, C A" sort="Reddy, C A" uniqKey="Reddy C" first="C A" last="Reddy">C A Reddy</name></author><author><name sortKey="Grethlein, H 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scheme="KwdFr" xml:lang="fr"><term>Bioréacteurs (MeSH)</term><term>Chromatographie gazeuse-spectrométrie de masse (MeSH)</term><term>Dialyse (méthodes)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Eau (MeSH)</term><term>Lignine (composition chimique)</term><term>Lignine (métabolisme)</term><term>Logiciel (MeSH)</term><term>Matrice extracellulaire (enzymologie)</term><term>Peroxidases (composition chimique)</term><term>Peroxidases (métabolisme)</term><term>Phanerochaete (enzymologie)</term><term>Solubilité (MeSH)</term><term>Spectroscopie infrarouge à transformée de Fourier (méthodes)</term><term>Traitement d'image par ordinateur (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Lignin</term><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Lignine</term><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Matrice extracellulaire</term><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Extracellular Matrix</term><term>Phanerochaete</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Dialysis</term><term>Spectroscopy, Fourier Transform Infrared</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lignine</term><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Dialyse</term><term>Spectroscopie infrarouge à transformée de Fourier</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Bioreactors</term><term>Gas Chromatography-Mass Spectrometry</term><term>Image Processing, 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However, their ability to degrade insoluble natural lignin in aqueous media has not been demonstrated. Insoluble isolated poplar lignin similar to natural lignin was treated in vitro in aqueous media for 12 h with LIP, MNP, and both. Treatment with MNP alone slightly increased the solid mass and produced measurable amounts of lignin-derived 2,6-dimethoxyhydroquinone and 2-methoxyhydroquinone but did not appreciably decrease the total lignin content. Treatment with LIP alone did not decrease the mass but produced measurable amounts of lignin-derived p-hydroxybenzoic acid and slightly decreased the lignin content. Finally, treatment with LIP and MNP together decreased the solid mass by 11%, decreased the lignin content by 5%, and released low-concentration compounds with mass spectra containing the typical lignin-derived electron-impact fragments of mass 107, 137, 151, 167, and 181. These results suggest that MNP increases the effectiveness of LIP-mediated lignin degradation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10099250</PMID><DateCompleted><Year>1999</Year><Month>05</Month><Day>03</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0006-3592</ISSN><JournalIssue CitedMedium="Print"><Volume>57</Volume><Issue>6</Issue><PubDate><Year>1998</Year><Month>Mar</Month><Day>20</Day></PubDate></JournalIssue><Title>Biotechnology and bioengineering</Title><ISOAbbreviation>Biotechnol Bioeng</ISOAbbreviation></Journal><ArticleTitle>In vitro degradation of natural insoluble lignin in aqueous media by the extracellular peroxidases of Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>704-17</MedlinePgn></Pagination><Abstract><AbstractText>The lignin peroxidases (LIP) and manganese peroxidases (MNP) of Phanerochaete chrysosporium catalyze a wide range of lignin depolymerization reactions with lignin models and synthetic lignins in solution. However, their ability to degrade insoluble natural lignin in aqueous media has not been demonstrated. Insoluble isolated poplar lignin similar to natural lignin was treated in vitro in aqueous media for 12 h with LIP, MNP, and both. Treatment with MNP alone slightly increased the solid mass and produced measurable amounts of lignin-derived 2,6-dimethoxyhydroquinone and 2-methoxyhydroquinone but did not appreciably decrease the total lignin content. Treatment with LIP alone did not decrease the mass but produced measurable amounts of lignin-derived p-hydroxybenzoic acid and slightly decreased the lignin content. Finally, treatment with LIP and MNP together decreased the solid mass by 11%, decreased the lignin content by 5%, and released low-concentration compounds with mass spectra containing the typical lignin-derived electron-impact fragments of mass 107, 137, 151, 167, and 181. These results suggest that MNP increases the effectiveness of LIP-mediated lignin degradation.</AbstractText><CopyrightInformation>Copyright 1998 John Wiley & Sons, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Thompson</LastName><ForeName>D N</ForeName><Initials>DN</Initials><AffiliationInfo><Affiliation>Department of Chemical Engineering, Michigan State University, East Lansing, Michigan 48824, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hames</LastName><ForeName>B R</ForeName><Initials>BR</Initials></Author><Author ValidYN="Y"><LastName>Reddy</LastName><ForeName>C A</ForeName><Initials>CA</Initials></Author><Author ValidYN="Y"><LastName>Grethlein</LastName><ForeName>H E</ForeName><Initials>HE</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, 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MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019149" MajorTopicYN="N">Bioreactors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003956" MajorTopicYN="N">Dialysis</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005109" MajorTopicYN="N">Extracellular Matrix</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008401" MajorTopicYN="N">Gas Chromatography-Mass Spectrometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007091" MajorTopicYN="N">Image Processing, Computer-Assisted</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012984" MajorTopicYN="N">Software</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012995" MajorTopicYN="N">Solubility</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017550" MajorTopicYN="N">Spectroscopy, Fourier Transform Infrared</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014867" MajorTopicYN="N">Water</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1999</Year><Month>4</Month><Day>1</Day><Hour>3</Hour><Minute>8</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2000</Year><Month>6</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1999</Year><Month>4</Month><Day>1</Day><Hour>3</Hour><Minute>8</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">10099250</ArticleId><ArticleId IdType="pii">10.1002/(SICI)1097-0290(19980320)57:6<704::AID-BIT8>3.0.CO;2-P</ArticleId><ArticleId IdType="doi">10.1002/(sici)1097-0290(19980320)57:6<704::aid-bit8>3.0.co;2-p</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Michigan</li></region><settlement><li>East Lansing</li></settlement><orgName><li>Université d'État du Michigan</li></orgName></list><tree><noCountry><name sortKey="Grethlein, H E" sort="Grethlein, H E" uniqKey="Grethlein H" first="H E" last="Grethlein">H E Grethlein</name><name sortKey="Hames, B R" sort="Hames, B R" uniqKey="Hames B" first="B R" last="Hames">B R Hames</name><name sortKey="Reddy, C A" sort="Reddy, C A" uniqKey="Reddy C" first="C A" last="Reddy">C A Reddy</name></noCountry><country name="États-Unis"><region name="Michigan"><name sortKey="Thompson, D N" sort="Thompson, D N" uniqKey="Thompson D" first="D N" last="Thompson">D N Thompson</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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